21+ Neb Ligation Calculator

Select thermostable ligase and reaction buffer. Convert double strand DNA ds DNA to pmoles and ends.

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Are you carrying out the ligation reaction under high salt conditions 100 mM NaCl.

. For example in NEBuffer 31. The NEBioCalculator Ligation module can help you plan a successful ligation reaction as part of a restriction cloning workflow by optimizing the molar ratio between your vector and insert. Our ligation molar ratio calculator uses the following equation.

Instructions Select the product group of the polymerase or kit you plan to use. Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products. This cookie is set by GDPR Cookie.

Single base 1 nt TA overhangs. Use this tool to estimate an appropriate incubation temperature when using NEB thermostable ligases and reaction buffers. Ds DNA size in base pairs bp Quantity in micogram ug PART 2.

This tutorial describes the use of the NEBioCalculator web tool to optimize the molar ratio between vector and insert DNA for use in a ligation reaction. If your insert is smaller than the vector say if youre trying to ligate a 1kb insert into a 3kb vector youll need a higher ratio in this case about a 31 molar ratio of insert to vector. Set by the GDPR Cookie Consent plugin this cookie is used to record the user consent for the cookies in the Advertisement category.

Required insert mass Vector mass Insert lengthVector length Molar ratio where. NEBioCalculator - Using the Ligation module This tutorial describes the use of the NEBioCalculator web tool to optimize the molar ratio between vector and insert DNA for use in.

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